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GSM signal) does not Affect Micronucleus Frequency and cytokinesis-block micronucleus assay. .. total micronuclei) obtained at the ENEA and IREA labo-. The frequency of micronuclei (MN) in peripheral blood lymphocytes (PBL) is extensively used as a biomarker of chromosomal damage and genome stabilit. Naples, Italy @ Abstract. The cytokinesis-block micronucleus assay is a sensitive and simple indicator of chromosome damage, both.

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The follow-up period began with the date of MN testing and ended with death, cancer diagnosis, emigration, 85th birthday or end of follow-up —, depending on the countrywhichever occurred first. B A photomicrograph of a mitogen-stimulated, cytokinesis-blocked lymphocyte containing one MN.

Furthermore, we stratified the entire cohort according to the median follow-up time among the cancer cases, i. To verify if the presence of pre-clinical stages of cancer might have influenced MN frequency at test we repeated the analyses excluding the first 2 years of follow-up. Only findings from Japan, which is the oldest national cohort and has the largest number of events, reached statistical significance, i.

The cohorts investigated in this study were different from those that were previously studied to determine the relationship between CA in PBL and cancer risk 15— Only individual MN frequencies based on the scoring of at least interphase cells were considered for statistical analysis. The results from the present study provide preliminary evidence that MN frequency in PBL is a predictive biomarker of cancer risk within a population of healthy subjects.

Occupational exposure to mutagens or smoking status did not significantly modify the relationship between MN frequency and cancer risk. In order to standardize for the marked inter-laboratory variability see Table I MN frequency was categorized by tertiles of the laboratory-specific distributions, i. Much theoretical evidence has been accumulated supporting the causal role of MN induction in cancer development, although prospective cohort studies are needed to validate MN as a cancer risk biomarker.


The original cytogenetic studies were performed between andand most of them have been published in the peer-reviewed scientific literature. Registration Forgot your password? Morley mitosis CYT-B: Induced detachment of acentric chromatin from mitotic chromosomes leads to their cytoplasmic localization at G 1 and the micronucleation by lamin reorganization at S phase. Latest Most Read Most Cited Genetic variants in the calcium signaling pathway genes are associated with cutaneous melanoma-specific survival.

OK Laboratory of Radiation Biology. Mitosis is just one part of the cell cycle The Mitotic M phase is the shortest part of the cell cycle Cytokinesis may be included. Twenty-one subjects diagnosed with a non-melanoma skin cancer ICD-IX were excluded from the analyses. This distribution in presence of overdispersion, a phenomenon that frequently arises with count data, provides more efficient estimates of the standard errors of the models parameters.

Oligomeric proanthocyanidins OPCs from grape seed extract suppress the activity of ABC transporters in overcoming chemoresistance in colorectal cancer cells.

Institute for Electromagnetic Sensing of Environment Naples, Italy

Collection of 1 ml culture Centrifugation at rpm for 1 minute Red cells break lysis buffer for 7 minutes 3 washing steps Hypotonic treatment 15 min. The authors are grateful to Dr Kei Nakachi, Hiroshima, Japan for his constructive comments on the manuscript.

However, despite the uneven size of the micronkclei, the increased RR’s in all national cohorts, as shown in Table IIIadds confidence to the reliability of the statistical model used for the overall analysis. Chromosomal aberrations and cancer risk: EDTA disodium salt 0.

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We think you have liked this presentation. Information on cancer incidence was obtained by linking the cohorts with national or regional cancer registries. Telomere dysfunction and evolution of intestinal carcinoma in mice and humans. STATA software was used for all statistical analyses Data on smoking status at the moment of cytogenetic testing were available in all cohorts, and subjects were classified as current, former or never smoker without consideration of the level of smoking.

While a residual confounding due to occupational exposure to mutagens or smoking status is still possible as shown by the small difference between adjusted and unadjusted relative risk estimatesstatistical analysis showed a lack of effect modification, i.

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All these potential sources of discrepancy—including the intrinsic limitation due to measurement of MN in a surrogate tissue—may have weakened the observed association between MN and cancer incidence. Evaluation of a potential mutagenic MOA based on analysis of the weight of evidence and using the modified Hill criteria Martha M. Nicronuclei are air dried, fixed and stained in a Giemsa solution.

Citing articles via Web of Science The results from the present study support the hypothesis that MN frequency in PBL is a predictive biomarker of cancer risk. If you wish to download it, please recommend it to your friends in any social system. Elimination of micronucleated cells by apoptosis after treatment with inhibitors of microtubules.